B-Raf Antibody (1H12F1)

Images

 
Western Blot: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis using BRAF mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).
Immunohistochemistry-Paraffin: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis of human bladder carcinoma tissue(left) and lung carcinoma tissue (right) showing cytoplasmic localization using BRAF mouse mAb with DAB ...read more
Western Blot: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis using BRAF mouse mAb against truncated recombinant Braf (1) and A431 cell lysate (2).
Immunohistochemistry-Paraffin: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis of human testis tissues using BRAF mouse mAb.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clone
1H12F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

B-Raf Antibody (1H12F1) Summary

Immunogen
Purified recombinant fragment of BRAF expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BRAF
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1: 500 - 1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for B-Raf Antibody (1H12F1)

  • 94 kDa B-raf protein
  • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
  • BRaf
  • B-Raf
  • BRAF1
  • B-RAF1
  • BRAF1MGC126806
  • EC 2.7.11.1
  • MGC138284
  • murine sarcoma viral (v-raf) oncogene homolog B1
  • NS7
  • p94
  • Proto-oncogene B-Raf
  • RAFB1
  • serine/threonine-protein kinase B-raf
  • v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Background

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00003845-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
AF482
Species: Mu
Applications: WB, IHC
MAB4540
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP2-46403
Species: Hu
Applications: IHC
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-94887
Species: Hu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-47945
Species: Hu, Mk
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-33415
Species: Hu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NBP1-47833
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45745
Species: Hu
Applications: WB, IHC, IF
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33581
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-33404
Species: Hu
Applications: IHC, IHC-P
NBP2-43792
Species: Hu
Applications: WB
NBP1-47555
Species: Hu
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P

Publications for B-Raf Antibody (NBP1-47555) (0)

There are no publications for B-Raf Antibody (NBP1-47555).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for B-Raf Antibody (NBP1-47555) (0)

There are no reviews for B-Raf Antibody (NBP1-47555). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for B-Raf Antibody (NBP1-47555) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional B-Raf Products

Bioinformatics Tool for B-Raf Antibody (NBP1-47555)

Discover related pathways, diseases and genes to B-Raf Antibody (NBP1-47555). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for B-Raf Antibody (NBP1-47555)

Discover more about diseases related to B-Raf Antibody (NBP1-47555).
 

Pathways for B-Raf Antibody (NBP1-47555)

View related products by pathway.

PTMs for B-Raf Antibody (NBP1-47555)

Learn more about PTMs related to B-Raf Antibody (NBP1-47555).
 

Research Areas for B-Raf Antibody (NBP1-47555)

Find related products by research area.

Blogs on B-Raf.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

You can't be without me - SNF5
The protein encoded by SNF5 is a component of the chromatin-remodeling protein complex responsible for relieving repressive chromatin structures by allowing the transcriptional machinery to access targets more effectively. SNF5 has been found to be a...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our B-Raf Antibody (1H12F1) and receive a gift card or discount.

Bioinformatics

Gene Symbol BRAF
Entrez