Axl [p Tyr779] Antibody

Images

 
Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with WI38 human lung cell line conditioned media (WI38 CM) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Human ...read more
Axl phosphorylated at Y779 (panels B, D) and total Axl (panels A, C) were assessed in immersion fixed A172 human glioblastoma cells incubated with (panels C, D) or without (panels A, B) pervanadate. Phospho-Axl was ...read more
Axl phosphorylated at site Y779 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Axl [p Tyr779] Antibody Summary

Immunogen
Phosphopeptide containing human Axl Y779 site
Modification
p Tyr779
Specificity
Detects human and mouse Axl when phosphorylated at Y779.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AXL
Purity
Antigen and protein A Affinity-purified
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2228 in the following applications:

Publications
Read Publications using
AF2228 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen and protein A Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axl [p Tyr779] Antibody

  • AI323647
  • ARK
  • AXL oncogene
  • AXL receptor tyrosine kinase
  • AXL transforming sequence/gene
  • Axl
  • EC 2.7.10
  • EC 2.7.10.1
  • JTK11
  • Tyro7
  • tyrosine-protein kinase receptor UFO
  • UFO
  • UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K‑dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Axl Antibody (AF2228)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(15)
All Applications
Filter By Species
Human
(15)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF2228 Applications Species
W Du, L Sun, T Liu, J Zhu, Y Zeng, Y Zhang, X Wang, Z Liu, JA Huang The miR?625?3p/AXL axis induces non?T790M acquired resistance to EGFR?TKI via activation of the TGF?&beta/Smad pathway and EMT in EGFR?mutant non?small cell lung cancer Oncol. Rep., 2020;0(0):. 2020 [PMID: 32319651] (Western Blot, Human) Western Blot Human
MT Kuo, Y Long, WB Tsai, YY Li, HHW Chen, LG Feun, N Savaraj Collaboration Between RSK-EphA2 and Gas6-Axl RTK Signaling in Arginine Starvation Response That Confers Resistance to EGFR Inhibitors Transl Oncol, 2019;13(2):355-364. 2019 [PMID: 31887630] (Western Blot, Human) Western Blot Human
JP Hoj, B Mayro, AM Pendergast A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis Cell Rep, 2019;29(11):3421-3434.e8. 2019 [PMID: 31825826] (Western Blot, Mouse) Western Blot Mouse
N Al Kafri, S Hafizi Tumour-Secreted Protein S (ProS1) Activates a Tyro3-Erk Signalling Axis and Protects Cancer Cells from Apoptosis Cancers (Basel), 2019;11(12):. 2019 [PMID: 31766614] (Western Blot, Human) Western Blot Human
JWP Bracht, N Karachalio, J Berenguer, C Pedraz-Val, M Filipska, C Codony-Ser, J Codony-Ser, R Rosell Osimertinib and pterostilbene in EGFR-mutation-positive non-small cell lung cancer (NSCLC) Int. J. Biol. Sci., 2019;15(12):2607-2614. 2019 [PMID: 31754333] (Western Blot, Human) Western Blot Human
W Li, A Dick, F Lu, H Zhang, H Sun Induction of MET Receptor Tyrosine Kinase Down-regulation through Antibody-mediated Receptor Clustering Sci Rep, 2019;9(1):1988. 2019 [PMID: 30760737] (Western Blot, Human) Western Blot Human
LC Cheng, YL Chen, AN Cheng, AY Lee, CY Cho, JS Huang, SE Chuang AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells J. Biomed. Sci., 2018;25(1):80. 2018 [PMID: 30419905] (Western Blot, Human) Western Blot Human
S Maacha, J Hong, A von Lersne, A Zijlstra, A Belkhiri AXL Mediates Esophageal Adenocarcinoma Cell Invasion through Regulation of Extracellular Acidification and Lysosome Trafficking Neoplasia, 2018;20(10):1008-1022. 2018 [PMID: 30189359] (Western Blot, Human) Western Blot Human
B Leonard, TM Brand, RA O'Keefe, E Lee, Y Zang, JD Kemmer, H Li, JR Grandis, NE Bhola BET inhibition overcomes receptor tyrosine kinase-mediated cetuximab resistance in HNSCC Cancer Res., 2018;0(0):. 2018 [PMID: 29792310] (Western Blot, Human) Western Blot Human
YL Chen, YT Tsai, TT Chao, YN Wu, MC Chen, YH Lin, CH Liao, SP Chou, HS Chiang DAPK and CIP2A are involved in GAS6/AXL-mediated Schwann cell proliferation in a rat model of bilateral cavernous nerve injury Oncotarget, 2018;9(5):6402-6415. 2018 [PMID: 29464081] (IHC-P, Rat) IHC-P Rat
Show All 18 Publications.

Review for Axl Antibody (AF2228) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2228:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IHC-P Human 01/06/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee Pmid 24687913
SpeciesHuman

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Recent Reviews

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1
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Anonymous
01/06/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol AXL