Axl [p Tyr779] Antibody

Images

 
Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with WI38 human lung cell line conditioned media (WI38 CM) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Human ...read more
Axl phosphorylated at Y779 (panels B, D) and total Axl (panels A, C) were assessed in immersion fixed A172 human glioblastoma cells incubated with (panels C, D) or without (panels A, B) pervanadate. Phospho-Axl was ...read more
Axl phosphorylated at site Y779 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Axl [p Tyr779] Antibody Summary

Immunogen
Phosphopeptide containing human Axl Y779 site
Modification
p Tyr779
Specificity
Detects human and mouse Axl when phosphorylated at Y779.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AXL
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2228 in the following applications:

Publications
Read Publications using
AF2228 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen and protein A Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axl [p Tyr779] Antibody

  • Ark
  • AXL oncogene
  • AXL receptor tyrosine kinase
  • AXL transforming sequence/gene
  • Axl
  • EC 2.7.10
  • EC 2.7.10.1
  • JTK11
  • tyrosine-protein kinase receptor UFO
  • Ufo
  • UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K‑dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-28635
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, Flow-IC
AF591
Species: Mu
Applications: Flow, IHC, CyTOF-ready
NLS4271
Species: Hu, Rt, Mk, Pm
Applications: WB, IHC, IHC-P, IF
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
H00054778-M05
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-91258
Species: Hu, Mu, Bv, Ca, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF527
Species: Mu
Applications: WB, IHC, Block, ICC
NBP1-80841
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-19804
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-38464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-67471
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF2228
Species: Hu
Applications: WB, IHC, ICC/IF

Publications for Axl Antibody (AF2228)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC - Paraffin embedded, IHC Frozen, IP, WB.


Filter By Application
IHC - Paraffin embedded
(1)
IHC Frozen
(1)
IP
(1)
WB
(7)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF2228 Applications Species
B Leonard, TM Brand, RA O'Keefe, E Lee, Y Zang, JD Kemmer, H Li, JR Grandis, NE Bhola BET inhibition overcomes receptor tyrosine kinase-mediated cetuximab resistance in HNSCC Cancer Res., 2018;0(0):. 2018 [PMID: 29792310] (WB,, Human) WB Human
YL Chen, YT Tsai, TT Chao, YN Wu, MC Chen, YH Lin, CH Liao, SP Chou, HS Chiang DAPK and CIP2A are involved in GAS6/AXL-mediated Schwann cell proliferation in a rat model of bilateral cavernous nerve injury Oncotarget, 2018;9(5):6402-6415. 2018 [PMID: 29464081] (IHC - Paraffin embedded,, Rat) IHC - Paraffin embedded Rat
K Iida, R Sakai, S Yokoyama, N Kobayashi, S Togo, HY Yoshikawa, A Rawangkan, K Namiki, M Suganuma Cell softening in malignant progression of human lung cancer cells by activation of receptor tyrosine kinase AXL Sci Rep, 2017;7(1):17770. 2017 [PMID: 29259259] (WB,, Human) WB Human
H Shin, D Kim, DM Helfman Tropomyosin isoform Tpm2.1 regulates collective and amoeboid cell migration and cell aggregation in breast epithelial cells Oncotarget, 2017;8(56):95192-95205. 2017 [PMID: 29221121] (WB,, Human) WB Human
C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma Br. J. Cancer, 2017;117(12):1787-1797. 2017 [PMID: 29024938] (WB,, Human) WB Human
R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy Sci Rep, 2017;7(1):10613. 2017 [PMID: 28878389] (WB,, Human) WB Human
Park I, Mundy-Bosse B, Whitman S, Zhang X, Warner S, Bearss D, Blum W, Marcucci G, Caligiuri M Receptor tyrosine kinase Axl is required for resistance of leukemic cells to FLT3-targeted therapy in acute myeloid leukemia. Leukemia, 2015;29(12):2382-9. 2015 [PMID: 26172401] (WB,, Human) WB Human
Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. 2012 [PMID: 23071254] (IHC Frozen,, Human) IHC Frozen Human
Ghosh AK, Secreto C, Boysen J, Sassoon T, Shanafelt TD, Mukhopadhyay D, Kay NE The novel receptor tyrosine kinase Axl is constitutively active in B-cell chronic lymphocytic leukemia and acts as a docking site of nonreceptor kinases: implications for therapy. Blood, 2011;117(6):1928-37. 2011 [PMID: 21135257] (IP,, Human) IP Human
Lafdil F, Chobert MN, Deveaux V, Zafrani ES, Mavier P, Nakano T, Laperche Y, Brouillet A Growth arrest-specific protein 6 deficiency impairs liver tissue repair after acute toxic hepatitis in mice. J. Hepatol., 2009;51(1):55-66. 2009 [PMID: 19443073] (WB,, Mouse) WB Mouse

Review for Axl Antibody (AF2228) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2228:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 01/06/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee Pmid 24687913
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Axl Antibody (AF2228) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Axl Antibody (AF2228)

Discover related pathways, diseases and genes to Axl Antibody (AF2228). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Axl Antibody (AF2228)

Discover more about diseases related to Axl Antibody (AF2228).
 

Pathways for Axl Antibody (AF2228)

View related products by pathway.

PTMs for Axl Antibody (AF2228)

Learn more about PTMs related to Axl Antibody (AF2228).

Blogs on Axl

There are no specific blogs for Axl, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
01/06/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol AXL