Axl Antibody (6C8)

Western Blot: Axl Antibody (6C8) [H00000558-M01] - Analysis of AXL expression in transfected 293T cell line by AXL monoclonal antibody (M01), clone 6C8.Lane 1: AXL transfected lysate(98 KDa).Lane 2: Non-transfected lysate.

Western Blot: Axl Antibody (6C8) [H00000558-M01] - Analysis of AXL over-expressed 293 cell line, cotransfected with AXL Validated Chimera RNAi ( Cat # H00000558-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AXL monoclonal antibody (M01) clone 6C8 (Cat # H00000558-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Axl Antibody (6C8) [H00000558-M01] - AXL monoclonal antibody (M01), clone 6C8 Analysis of AXL expression in HeLa.

ELISA: Axl Antibody (6C8) [H00000558-M01] - Detection limit for recombinant GST tagged AXL is approximately 0.3ng/ml as a capture antibody.

Product Discontinued

Axl Antibody (6C8) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: AXL (AAH32229, 30 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TQAEESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQVPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPY
• Specificity: AXL - AXL receptor tyrosine kinase
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AXL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Axl Antibody (6C8)

• AI323647
• ARK
• AXL oncogene
• AXL receptor tyrosine kinase
• AXL transforming sequence/gene
• Axl
• EC 2.7.10
• EC 2.7.10.1
• JTK11
• Tyro7
• tyrosine-protein kinase receptor UFO
• UFO
• UFOoncogene AXL

Background

The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axl Antibody (H00000558-M01)(3)

Publications using H00000558-M01

• Ammoun S, Provenzano L, Zhou L et al. Axl/Gas6/NFkB signalling in schwannoma pathological proliferation, adhesion and survival. Oncogene. 2013-01-14 [PMID: 23318455]
• Vaughan CA, Singh S, Windle B et al. Gain-of-Function Activity of Mutant p53 in Lung Cancer through Up-Regulation of Receptor Protein Tyrosine Kinase Axl. Genes & Cancer. 2012-07-01 [PMID: 23264849]
• Vaughan CA, Singh S, Windle B et al. Gain-of-Function Activity of Mutant p53 in Lung Cancer through Up-Regulation of Receptor Protein Tyrosine Kinase Axl. Genes & Cancer

Bioinformatics

• Gene Symbol: AXL
• Entrez:
  • 558(Human)
• OMIM:
  • 109135(Human)
• Uniprot:
  • AAH32229()