Aurora C Antibody (OTI2B7) - Azide and BSA Free

Western Blot: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aurora C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aurora C.

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded pancreas tissue using anti-Aurora C mouse monoclonal antibody.

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aurora C mouse monoclonal antibody.

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Aurora C mouse monoclonal antibody.

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded bladder tissue using anti-Aurora C mouse monoclonal antibody.

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-Aurora C mouse monoclonal antibody.

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded colon tissue using anti-Aurora C mouse monoclonal antibody.

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded endometrium tissue using anti-Aurora C mouse monoclonal antibody.

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded prostate tissue using anti-Aurora C mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI2B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Aurora C Antibody (OTI2B7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Aurora C Antibody (OTI2B7) - Azide and BSA Free (NBP1-47663) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human AURKC expression vector
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AURKC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200 - 1:1000
• Theoretical MW: 35.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Aurora C Antibody (OTI2B7) - Azide and BSA Free

• AIE2
• AIE2AIK3
• AIK3
• AIRK3
• ARK3
• ARK-3
• ARK3Serine/threonine-protein kinase aurora-C
• AurC
• AURKC
• Aurora 3
• Aurora C
• aurora kinase CAurora/IPL1/Eg2 protein 2
• Aurora/IPL1-related kinase 3
• aurora-C
• Aurora-related kinase 3
• EC 2.7.11
• EC 2.7.11.1
• serine/threonine kinase 13 (aurora/IPL1-like)
• serine/threonine-protein kinase 13
• STK13
• STK13ARK-3

Background

Aurora-C is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process (1). Studies show that human Aurora-C is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere protein (INCENP), which are known passenger proteins. INCENP binds and activates Aurora-C in vivo and in vitro. Aurora-C co-expressed with INCENP elicits the phosphorylation of endogenous histone H3 in mammalian cells, even though this phosphorylation is not sufficient to establish chromosome condensation in interphase cells (2). Over-expression of an inactive mutant of Aurora C impaired the localization of Aurora C to the spindle midzone resulting in multinucleation which demonstrates that Aurora C is required for cytokinesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AURKC