Aurora B Antibody (rAURKB/1592)

Images

 
Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) [NBP3-07553] - Formalin-fixed, paraffin-embedded human placenta stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).
Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) [NBP3-07553] - Formalin-fixed, paraffin-embedded human kidney stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).
Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) [NBP3-07553] - Formalin-fixed, paraffin-embedded human kidney stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P
Clone
rAURKB/1592
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Aurora B Antibody (rAURKB/1592) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary) (Uniprot: Q96GD4)
Localization
Nuclear and Cytoplasmic
Marker
Proliferation Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AURKB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08877)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Aurora B Antibody (rAURKB/1592)

  • Aik2
  • AIK2EC 2.7.11.1
  • AIM1
  • AIM-1
  • AIM1Aik2
  • AIM-1STK-1
  • ARK2
  • ARK2ARK-2
  • AurB
  • AURKB
  • aurkb-sv2
  • Aurora- and IPL1-like midbody-associated protein 1
  • Aurora B
  • aurora kinase BSerine/threonine-protein kinase aurora-B
  • aurora kinase B-Sv1
  • aurora kinase B-Sv2
  • Aurora/IPL1-related kinase 2
  • aurora-1
  • aurora-B
  • Aurora-related kinase 2
  • EC 2.7.11
  • IPL1
  • serine/threonine kinase 12
  • serine/threonine-protein kinase 12
  • STK12
  • STK12aurkb-sv1
  • STK5

Background

Aurora-B is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process. Aurora-B is known to be involved in the regulation of centrosome function, bipolar spindle assembly and chromosome segregation. Aurora kinases are localized at the centrosomes of interphase cells, at the poles of the bipolar spindle and in the midbody of the mitotic apparatus. Substrates identified for the Aurora-B kinases include a kinesin-like motor protein, spindle apparatus proteins, histone H3 protein, kinetochore protein and the tumor suppressor protein p53 (1). Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein, also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AURKB
Uniprot