Aurora A Antibody (35C1) [Biotin]

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Western Blot: Aurora A Antibody (35C1) [Biotin] [NB100-1829] - Western blot analysis of recombinant Aurora-A kinase protein probed with 100-1829 and F(ab')2 rabbit anti-mouse IgG:HRP.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IP
Clone
35C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

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Catalog# & Conjugate Size Price

Aurora A Antibody (35C1) [Biotin] Summary

Immunogen
Recombinant full length his-tagged Aurora A protein (Human).
Marker
Mitosis Marker
Specificity
Recognizes human Aurora-A kinase, a ~46kDa serine/threonine kinase involved in mitotic spindle assembly. Clone 35C1 specifically recognises an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
AURKA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
Positive Control
Aurora A Lysate (NBL1-07855)
Publications
Read Publication using NB100-1829.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Bovine reactivity reported in scientific literature (PMID: 26168150)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Aurora A Antibody (35C1) [Biotin]

  • AIK
  • AIKAurA
  • ARK1
  • ARK-1
  • ARK1EC 2.7.11.1
  • AURA
  • AURKA
  • Aurora A
  • aurora kinase AhARK1
  • aurora/IPL1-like kinase
  • Aurora/IPL1-related kinase 1
  • Aurora-related kinase 1
  • Breast tumor-amplified kinase
  • breast-tumor-amplified kinase
  • BTAK
  • BTAKAURORA2
  • EC 2.7.11
  • IPL1-related kinase
  • serine/threonine kinase 6
  • serine/threonine protein kinase 15
  • Serine/threonine-protein kinase 15
  • serine/threonine-protein kinase 6
  • Serine/threonine-protein kinase aurora-A
  • STK15
  • STK15serine/threonine kinase 15
  • STK6
  • STK6MGC34538
  • STK7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora A Antibody (NB100-1829)(1)


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Positive Control Lysate(s)

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Bioinformatics

Gene Symbol AURKA
Entrez