Aurora A Antibody (35C1) - BSA Free

Western Blot: Aurora A Antibody (35C1) [NB100-1641] - Analysis using the Biotin conjugate of NB100-1641. Detection of recombinant Aurora-A kinase protein probed with 100-1829 and F(ab')2 rabbit anti-mouse IgG:HRP.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clone: 35C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Aurora A Antibody (35C1) - BSA Free Summary

• Immunogen: Recombinant Aurora-A
• Marker: Mitosis Marker
• Specificity: Recognizes human Aurora-A kinase, a ~46kDa serine/threonine kinase involved in mitotic spindle assembly. Clone 35C1 specifically recognises an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: AURKA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  Aurora A Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Bovine reactivity reported in scientific literature (PMID: 26168150)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Aurora A Antibody (35C1) - BSA Free

• AIK
• AIKAurA
• ARK1
• ARK-1
• ARK1EC 2.7.11.1
• AURA
• AURKA
• Aurora A
• aurora kinase AhARK1
• aurora/IPL1-like kinase
• Aurora/IPL1-related kinase 1
• Aurora-related kinase 1
• Breast tumor-amplified kinase
• breast-tumor-amplified kinase
• BTAK
• BTAKAURORA2
• EC 2.7.11
• IPL1-related kinase
• serine/threonine kinase 6
• serine/threonine protein kinase 15
• Serine/threonine-protein kinase 15
• serine/threonine-protein kinase 6
• Serine/threonine-protein kinase aurora-A
• STK15
• STK15serine/threonine kinase 15
• STK6
• STK6MGC34538
• STK7

Background

NB 100-1641 recognizes human Aurora-A kinase, a 46kDa serine-threonine kinase involved in mitotic spindle assembly. Aurora-A is reported to be overexpressed in many epitheial cancers and is thought to play an important for in tumorgenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aurora A Antibody (NB100-1641)(2)

Publications using NB100-1641

• Mahdipour M, Leitoguinho AR, Zacarias Silva RA et al. TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes PLoS ONE. 2015-07-14 [PMID: 26168150] (ICC/IF, WB, Bovine, Human)
• Cremet JY et al. Preparation characterization of a human aurora-A kinase monoclonal antibody. Mol Cell Biochem 243:123-31. 2003-01-01 [PMID: 12619897]

Bioinformatics

• Gene Symbol: AURKA
• Uniprot:
  • O14965()