Aurora A Antibody (1F8)

Images

 
Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Western blot analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate.
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Immunofluorescence analysis of Hela cells using Aurora A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Flow cytometric analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).
ELISA: Aurora A Antibody (1F8) [NBP2-22118] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, Rt, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
1F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Aurora A Antibody (1F8) Summary

Immunogen
Purified recombinant fragment of human Aurora A expressed in E. coli. [UniProt# O14965]
Marker
Mitosis Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AURKA
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
Application Notes
This Aurora A (1F8) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, monkey and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Aurora A Antibody (1F8)

  • AIK
  • AIKAurA
  • ARK1
  • ARK-1
  • ARK1EC 2.7.11.1
  • AURA
  • AURKA
  • Aurora A
  • aurora kinase AhARK1
  • aurora/IPL1-like kinase
  • Aurora/IPL1-related kinase 1
  • Aurora-related kinase 1
  • Breast tumor-amplified kinase
  • breast-tumor-amplified kinase
  • BTAK
  • BTAKAURORA2
  • EC 2.7.11
  • IPL1-related kinase
  • serine/threonine kinase 6
  • serine/threonine protein kinase 15
  • Serine/threonine-protein kinase 15
  • serine/threonine-protein kinase 6
  • Serine/threonine-protein kinase aurora-A
  • STK15
  • STK15serine/threonine kinase 15
  • STK6
  • STK6MGC34538
  • STK7

Background

Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aurora A Antibody (NBP2-22118) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AURKA
Entrez
Uniprot