AUF1 Antibody

Product Discontinued

AUF1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 46-98 of Human hnRPD.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNRNPD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of hnRPD (H79) pAb in extracts from 293 cells.
  Immunohistochemistry (IHC) analyzes of hnRPD (H79) pAb in paraffin-embedded human lung carcinoma tissue.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AUF1 Antibody

• AUF1A
• heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein1, 37kDa)
• heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA-binding protein1, 37kD)
• heterogeneous nuclear ribonucleoprotein D0
• hnRNPD0
• type A

Background

hnRNP D/AUF1 binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3' untranslated regions of many protooncogenes and cytokine mRNAs. It also binds to double and single stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single stranded nonmonotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. It may be involved in translationally coupled mRNA turnover (referenced from swissprot).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPD
• Entrez:
  • 3184(Human)
  • 79256(Rat)