Atrial Natriuretic Peptide/ANP Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Atrial Natriuretic Peptide/ANP Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Atrial Natriuretic Peptide/ANP
  Asn26-Tyr151
  Accession # P01160
• Specificity: Detects human Atrial Natriuretic Peptide/ANP in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Atrial Natriuretic Peptide/ANP Antibody [Alexa Fluor® 647]

• ANF
• ANP
• ANPatriopeptin
• ATFB6
• atrial natriuretic factor
• Atrial Natriuretic Peptide
• Cardionatrin
• CDD-ANF
• natriuretic peptide A
• natriuretic peptide precursor A
• NPPA
• PND
• PNDcardionatrin
• prepronatriodilatin

Background

ANP (Atril Natriuetic Peptide; also known as gamma -ANP, CDP/cardiodilatin-related peptide and CDD-ANF) is a secreted member of the natriuretic peptide family of molecules. It is expressed in multiple cell types, including atrial myocardiocytes, macrophages and select hypothalamic neurons. The C-terminus of ANP (or ANF) is reported to have a wide range of activities. It is best known to promote sodium and water excretion, but is also known to inhibit aldosterone and vasopressin secretion, while promoting testosterone and LH release. Human (pro)ANP is synthesized as a 153 amino acid (aa) preproprecursor that contains a 25 aa signal sequence, cardiodilatin-related peptide (aa 26-55), a prosequence (aa 56-123) and a C-terminal atrial natriuretic factor motif (aa 124-151) that may or may not be accompanied by a dibasic Arg fragment. Although multiple enzymes are reported to be active on proANP, transmembrane Corin would appear to be central to normal extracellular proteolytic processing and the generation of ANP. Notably, and depending upon the tissue, proANP can also be cleaved at alternate sites, including Ala120 and Arg126. There is at least one isoform variant that contains a 12 aa addition to the C-terminus of the proprecursor, resulting in an ANP that is 40 aa in length. Over aa 26-151 (proANP), human proANP shares 84% aa sequence identity with mouse proANP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.