ATR Antibody (1E9) - Azide and BSA Free

Immunohistochemistry-Paraffin: ATR Antibody (1E9) [H00000545-M03] - Analysis of monoclonal antibody to ATR on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml

• Reactivity: Hu, Rt, Ha
• Applications: WB, ELISA, IHC
• Clone: 1E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATR Antibody (1E9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ATR (NP_001175, 2545 a.a. ~ 2644 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DQREPLMSVLKTFLHDPLVEWSKPVKGHSKAPLNETGEVVNEKAKTHVLDIEQRLQGVIKTRNRVTGLPLSIEGHVHYLIQEATDENLLCQMYLGWTPYM
• Specificity: ATR - ataxia telangiectasia and Rad3 related (1E9)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Antibody reactivity Against Recombinant Protein with GST tag on ELISA. GST tag alone is used as a negative control. It has been used for IHC-P. WB usage was reported in scientific literature. WB reported in scientific literature (PMID: 23451157). Use in Immunohistochemistry reported in scientific literature (PMID: 30746633).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29396668). Hamster reported in scientific literature (PMID: 23451157).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATR Antibody (1E9) - Azide and BSA Free

• ataxia telangiectasia and Rad3 related
• Ataxia telangiectasia and Rad3-related protein
• ATR
• EC 2.7.11.1
• FRAP-related protein 1
• FRP1
• FRP1FRAP-related protein-1
• MEC1
• protein kinase ATR
• Rad3 related protein
• SCKL1
• SCKLMEC1, mitosis entry checkpoint 1, homolog
• serine/threonine-protein kinase ATR

Background

The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATR Antibody (H00000545-M03)(5)

Publications using H00000545-M03

• Savva C, De Souza K, Ali R et al. Clinicopathological significance of ataxia telangiectasia-mutated (ATM) kinase and ataxia telangiectasia-mutated and Rad3-related (ATR) kinase in MYC overexpressed breast cancers Breast Cancer Res. Treat. 2019-02-12 [PMID: 30746633] (IF/IHC, Human)
• Al-Subhi N, Ali R, Abdel-Fatah T, Moseley PM. Targeting ataxia telangiectasia-mutated- and Rad3-related kinase (ATR) in PTEN-deficient breast cancers for personalized therapy. Breast Cancer Res. Treat. 2018-02-02 [PMID: 29396668] (Rat)
• Abdel-Fatah Tm, Middleton Fk, Arora A et al. Untangling the ATR-CHEK1 network for prognostication, prediction and therapeutic target validation in breast cancer. Mol Oncol. 2014-11-06 [PMID: 25468710] (IHC-P, Human)
• Abdel-Fatah TM, Perry C, Arora A et al. Is there a role for base excision repair in estrogen driven breast cancers? Antioxid. Redox Signal. 2014-08-11 [PMID: 25111287] (IHC-P, Human)
  
  Details:
  Rabbit polyclonal anti-APE1 antibody used for IHC on tissue microarray (TMAs) at a dilution of 1:500, incubated for 1 hour. Citrate buffer (pH6.0) was used as an antigen retrieval reagent (Table 3).
• Sultana R, Abdel-Fatah T, Perry C et al. Ataxia Telangiectasia Mutated and Rad3 Related (ATR) Protein Kinase Inhibition Is Synthetically Lethal in XRCC1 Deficient Ovarian Cancer Cells. PLoS One 2013-01-01 [PMID: 23451157] (WB, Hamster)

Bioinformatics

• Gene Symbol: ATR
• Entrez:
  • 545(Human)
• Uniprot:
  • NP_001175(Human)