ATPB Antibody (OTI4E5)

Western Blot: ATPB Antibody (4E5) [NBP2-02249] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATPB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATPB.

Immunocytochemistry/Immunofluorescence: ATPB Antibody (4E5) [NBP2-02249] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATPB.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded pancreas tissue using anti-ATPB mouse monoclonal antibody.

Flow Cytometry: ATPB Antibody (4E5) [NBP2-02249] - Analysis of Jurkat cells, using anti-ATPB antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western Blot: ATPB Antibody (4E5) [NBP2-02249] Analysis of extracts (35ug) from 9 different cell lines by using anti-ATPB monoclonal antibody.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ATPBmouse monoclonal antibody.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded breast tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded endometrium tissue using anti-ATPB mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded liver tissue using anti-ATPBmouse monoclonal antibody.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded lung tissue using anti-ATPBmouse monoclonal antibody.

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Ovary tissue using anti-ATPB mouse monoclonal antibody.

Flow Cytometry: ATPB Antibody (4E5) [NBP2-02249] - Analysis of Hela cells, using anti-ATPB antibody, (Red) compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI4E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.9 mg/ml

ATPB Antibody (OTI4E5) Summary

• Description: Novus Biologicals Mouse ATPB Antibody (OTI4E5) (NBP2-02249) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP5F1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 56.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.9 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ATPB Antibody (OTI4E5)

• ATP synthase subunit beta, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• ATPMB
• ATPSBmitochondrial ATP synthase beta subunit
• EC 3.6.3
• EC 3.6.3.14
• MGC5231
• mitochondrial ATP synthetase, beta subunit

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5F1B
• Entrez:
  • 670(Human)