ATP7A Antibody

Images

 
Western Blot: ATP7A Antibody [NBP1-54906] - HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.0 ug/ml Peptide Concentration: 2.0 ug/ml lysate Quantity: 25ug/ ...read more
Immunohistochemistry: ATP7A Antibody [NBP1-54906] - Human alveolar cell Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X.
Western Blot: ATP7A Antibody [NBP1-54906] - HepG2 cell lysate, Antibody Titration: 0.5ug/ml
Immunohistochemistry-Paraffin: ATP7A Antibody [NBP1-54906] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATP7A Antibody Summary

Immunogen
Synthetic peptides corresponding to ATP7A (ATPase, Cu++ transporting, alpha polypeptide (Menkes syndrome)) The peptide sequence was selected from the N terminal of ATP7A. Peptide sequence MKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP7A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against ATP7A and was validated on Western Blot and immunohistochemistry-P
Theoretical MW
163 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ATP7A Protein (NBP1-54906PEP)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for ATP7A Antibody

  • ATPase, Cu++ transporting, alpha polypeptide
  • Copper pump 1
  • copper-transporting ATPase 1
  • Cu++-transporting P-type ATPase
  • DSMAX
  • EC 3.6.3
  • EC 3.6.3.4
  • MC1
  • Menkes disease-associated protein
  • Menkes syndrome
  • MK
  • MNKFLJ17790
  • OHS
  • SMAX3

Background

The ATP7A gene encodes the Menkes copper-translocating P-type ATPase, a ubiquitous protein that regulates the absorption of copper in the gastrointestinal tract. Inside cells, this protein has a dual function: it delivers copper to cuproenzymes in the Golgi compartment and effluxes excess copper. The trafficking mechanism and catalytic activity combine to facilitate absorption and intercellular transport of copper. Menkes disease, a systemic copper deficiency disorder, is caused by mutations in the ATP7A gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP7A
Uniprot