ATP6V1B2 Antibody - BSA Free

Western Blot: ATP6V1B2 Antibody [NBP3-38048] - Western blot analysis of various lysates using ATP6V1B2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP6V1B2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATP6V1B2 Antibody - BSA Free (NBP3-38048) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 262-511 of human ATP6V1B2 (NP_001684.2).
  
  Sequence:
  NLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP6V1B2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ATP6V1B2 Antibody - BSA Free

• 000 subunit
• 56/58kD, isoform 2
• ATP6B2ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide
• ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Endomembrane proton pump 58 kDa subunit
• H+ transporting two-sector ATPase
• HO57ATP6B1B2
• vacuolar H+-ATPase 56
• Vacuolar proton pump subunit B 2
• VATB
• V-ATPase B2 subunit
• V-ATPase subunit B 2
• Vma2
• VPP3ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2
• V-type proton ATPase subunit B, brain isoform

Background

The ATP6V1B2 gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification ofeukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellularprocesses as protein sortin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP6V1B2