ATP6V1E1 Antibody

Images

 
Western Blot: ATP6V1E1 Antibody [NBP2-15520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATP6V1E1 antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: ATP6V1E1 Antibody [NBP2-15520] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-15520] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Western Blot: ATP6V1E1 Antibody [NBP2-15520] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE gel, diluted at 1:3000.
Western Blot: ATP6V1E1 Antibody [NBP2-15520] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATP6V1E1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ma-OpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATP6V1E1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ATP6V1E1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1E1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-15520 in the following applications:

  • WB
    1 publication

Reactivity Notes

Opossum reactivity reported in scientific literature (PMID: 28720662).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for ATP6V1E1 Antibody

  • ATP6E2ATP6V1E
  • ATP6E31kDa subunit
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD
  • ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1
  • ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
  • H+-transporting ATP synthase chain E, vacuolar
  • p31
  • Vacuolar proton pump subunit E 1
  • V-ATPase 31 kDa subunit
  • V-ATPase subunit E 1
  • V-ATPase, subunit E
  • Vma4
  • V-type proton ATPase subunit E 1

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ATP6V1E1 Antibody (NBP2-15520)(1)

We have publications tested in 1 confirmed species: Opossum.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ATP6V1E1
Uniprot