ATP6V1A Antibody (GT1561) - Azide and BSA Free

Western Blot: ATP6V1A Antibody (GT1561) [NBP3-13542] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT1561] (NBP3-13542) diluted at 1:500.

Flow Cytometry: ATP6V1A Antibody (GT1561) [NBP3-13542] - ATP6V1A antibody [GT1561] (NBP3-13542) detects ATP6V1A protein by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: ATP6V1A antibody [GT1561] (NBP3-13542) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.

Western Blot: ATP6V1A Antibody (GT1561) [NBP3-13542] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT1561] (NBP3-13542) diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM, Xp
• Applications: WB, Flow
• Clone: GT1561
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATP6V1A Antibody (GT1561) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.
• Predicted Species: Mouse (96%), Rat (95%), Porcine (96%), Bovine (98%), Rhesus Macaque (99%), Xenopus (90%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP6V1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Western Blot

Reactivity Notes

Chicken (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for ATP6V1A Antibody (GT1561) - Azide and BSA Free

• 70kD, isoform 1
• ATP6A1
• ATP6V1A
• ATP6V1A1
• ATP6V1A1ATPase, H+ transporting, lysosomal, subunit A1
• ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha polypeptide
• ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• EC 3.6.3
• EC 3.6.3.14
• EC 7.1.2.2
• H(+)-transporting two-sector ATPase, subunit A
• H+-transporting ATPase chain A, vacuolar (VA68 type)
• HO68
• VA68
• vacuolar ATP synthase catalytic subunit A, ubiquitous isoform
• Vacuolar ATPase isoform VA68
• vacuolar proton pump alpha subunit 1
• Vacuolar proton pump subunit alpha
• V-ATPase 69 kDa subunit 1
• V-ATPase 69 kDa subunit
• V-ATPase A subunit 1
• V-ATPase subunit A
• Vma1
• VPP2
• V-type proton ATPase catalytic subunit A

Background

ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP6V1A