ATP6V1A Antibody (4F5) - Azide and BSA Free

Western Blot: ATP6V1A Antibody (4F5) [H00000523-M02] - Analysis of ATP6V1A expression in transfected 293T cell line by ATP6V1A monoclonal antibody (M02), clone 4F5.Lane 1: ATP6V1A transfected lysate (Predicted MW: 68.3 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: ATP6V1A Antibody (4F5) [H00000523-M02] - Analysis of monoclonal antibody to ATP6V1A on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: ATP6V1A Antibody (4F5) [H00000523-M02] - Analysis of monoclonal antibody to ATP6V1A on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

Western Blot: ATP6V1A Antibody (4F5) [H00000523-M02] - ATP6V1A monoclonal antibody (M02), clone 4F5. Analysis of ATP6V1A expression in human kidney.

ELISA: ATP6V1A Antibody (4F5) [H00000523-M02] - Detection limit for recombinant GST tagged ATP6V1A is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATP6V1A Antibody (4F5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ATP6V1A (NP_001681, 508 a.a. ~ 617 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED
• Specificity: Reacts with ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP6V1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in WB and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATP6V1A Antibody (4F5) - Azide and BSA Free

• 70kD, isoform 1
• ATP6A1
• ATP6V1A
• ATP6V1A1
• ATP6V1A1ATPase, H+ transporting, lysosomal, subunit A1
• ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha polypeptide
• ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• EC 3.6.3
• EC 3.6.3.14
• EC 7.1.2.2
• H(+)-transporting two-sector ATPase, subunit A
• H+-transporting ATPase chain A, vacuolar (VA68 type)
• HO68
• VA68
• vacuolar ATP synthase catalytic subunit A, ubiquitous isoform
• Vacuolar ATPase isoform VA68
• vacuolar proton pump alpha subunit 1
• Vacuolar proton pump subunit alpha
• V-ATPase 69 kDa subunit 1
• V-ATPase 69 kDa subunit
• V-ATPase A subunit 1
• V-ATPase subunit A
• Vma1
• VPP2
• V-type proton ATPase catalytic subunit A

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATP6V1A Antibody (H00000523-M02)(3)

Publications using H00000523-M02

• Bhairavi T, Anna C, Yanmin Y et al. Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice. Nat Biotechnol. 2022-07-25 [PMID: 35879364]
• Ramirez C, Hauser A, Vucic E et al. Plasma membrane V-ATPase controls oncogenic RAS-induced macropinocytosis. Nature. 2019-12-11 [PMID: 31827278]
• Smith GA, Howell GJ, Phillips C et al. Extracellular and Luminal pH Regulation by Vacuolar H+-ATPase Isoform Expression and Targeting to the Plasma Membrane and Endosomes. J Biol Chem 2016-04-15 [PMID: 26912656]

Bioinformatics

• Gene Symbol: ATP6V1A
• Entrez:
  • 523(Human)
• Uniprot:
  • NP_001681(Human)