ATP5J Antibody (HL2934)

Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody [HL2934] (NBP3-48753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ATP5J antibody [HL2934] detects ATP5J protein by immunohistochemical analysis.Sample: Paraffin-embedded human heart.ATP5J stained by ATP5J antibody [HL2934] (NBP3-48753) diluted at 1:200.Antigen Retrieval: Tris-EDTA-Citrate buffer, pH 7.8, 15 min

ATP5J antibody [HL2934] detects ATP5J protein by immunohistochemical analysis.Sample: Paraffin-embedded human glioblastoma.ATP5J stained by ATP5J antibody [HL2934] (NBP3-48753) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody [HL2934] (NBP3-48753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL2934
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ATP5J Antibody (HL2934) Summary

• Description: Novus Biologicals Rabbit ATP5J Antibody (HL2934) (NBP3-48753) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full length human ATP5J recombinant protein.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATP5J
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for ATP5J Antibody (HL2934)

• ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
• ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
• ATP5
• ATP5A
• ATPase subunit F6
• ATPM
• coupling factor 6
• F6
• mitochondrial ATP synthase, coupling factor 6
• mitochondrial ATP synthase, subunit F6
• mitochondrial ATPase coupling factor 6
• mitochondrial
• proliferation-inducing protein 36

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the F0 complex, required for F1 and F0 interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5J