ATP5H Antibody - BSA Free

Immunoprecipitation:ATP5H AntibodyNBP3-30218] -Detection of human ATP5H by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP5H antibody NBP3-30218 (lot NBP3-30218-1) used for IP at 6 µg per reaction. ATP5H was also immunoprecipitated by rabbit anti-ATP5H.. For blotting immunoprecipitated ATP5H, NBP3-30218 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP5H Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATP5H Antibody - BSA Free (NBP3-30218) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 100 and 150
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP5PD
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for ATP5H Antibody - BSA Free

• ATP synthase D chain, mitochondrial
• ATP synthase subunit d, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
• ATP synthase, H+ transporting, mitochondrial F1F0, subunit d
• ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d
• ATP5JD
• ATPase subunit d
• ATPQ
• My032 protein

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the F0 complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5PD