NCALD Antibody - BSA Free

Western Blot: NCALD Antibody [NBP2-15037] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 12% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: NCALD Antibody [NBP2-15037] - Rat brain. Neurocalcin delta antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: NCALD Antibody [NBP2-15037] - Mouse brain. Neurocalcin delta antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, RM, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NCALD Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NCALD Antibody - BSA Free (NBP2-15037) is a polyclonal antibody validated for use in IHC and WB. Anti-NCALD Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NCALD. The exact sequence is proprietary.
• Predicted Species: Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (99%), Xenopus (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCALD
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NCALD Antibody - BSA Free

• MGC33870
• MGC74858
• neurocalcin delta
• neurocalcin-delta

Background

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NCALD Antibody (NBP2-15037)(1)

Publication using NBP2-15037

• Fukagawa T, Takafuji K, Tachibanaki S, Kawamura S. Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina. PLoS ONE. 2017-03-14 [PMID: 28291804] (Mouse)

Bioinformatics

• Gene Symbol: NCALD
• Uniprot:
  • P61601(Human)