ATP5H Antibody - BSA Free

Western Blot: ATP5H Antibody [NBP2-97512] - Anti-ATP5H rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: PC-3 Whole Cell Lysate. Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 20 kDa

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512] - Immunochemical staining of human ATP5H in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512] - Immunochemical staining of human ATP5H in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: ATP5H Antibody [NBP2-97512] - ATP5H was immunoprecipitated using: Lane A: 0.5 mg PC-3 Whole Cell Lysate. 4 ul anti-ATP5H rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ATP5H rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 22 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP5H Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human ATP5H fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP5PD
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation 0.5-2uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for ATP5H Antibody - BSA Free

• ATP synthase D chain, mitochondrial
• ATP synthase subunit d, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
• ATP synthase, H+ transporting, mitochondrial F1F0, subunit d
• ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d
• ATP5JD
• ATPase subunit d
• ATPQ
• My032 protein

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the F0 complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5PD