ATP5F1 Antibody

Images

 
Western Blot: ATP5F1 Antibody [NBP1-33465] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ATP5F1 Antibody [NBP1-33465] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ATP5F1 Antibody [NBP1-33465] - Paraffin-embedded colon; antibody dilution 1:500.
Western Blot: ATP5F1 Antibody [NBP1-33465] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.45 mg/ml

Order Details

ATP5F1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ATP synthase B1. The exact sequence is proprietary.
Localization
Mitochondrion, Mitochondrion inner membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP5F1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.45 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATP5F1 Antibody

  • ATP synthase B chain, mitochondrial
  • ATP synthase subunit b, mitochondrial
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
  • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1
  • ATPase subunit b
  • cell proliferation-inducing protein 47
  • H+-ATP synthase subunit b
  • MGC24431
  • PIG47

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP5F1