Recombinant Human ATP5A GST (N-Term) Protein

SDS-Page: ATP5A Recombinant Protein [H00000498-P01]

Product Discontinued

Recombinant Human ATP5A GST (N-Term) Protein Summary

• Description: Recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-553 of Human ATP5A1
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MLSVRVAAAVVRALPRRAGLVSRNALGSSFIAARNFHASNTHLQKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEIVTNFLAGFEA

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: ATP5F1A
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 86.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human ATP5A GST (N-Term) Protein

• ATP synthase alpha chain, mitochondrial
• ATP synthase subunit alpha, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 co
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2
• ATP sythase (F1-ATPase) alpha subunit
• ATP5A
• ATP5A1
• ATP5AL2
• ATP5AMOM2
• ATPM
• ATPMATP synthase subunit alpha, mitochondrial
• cardiac muscle
• COXPD22
• EC 3.6.3
• EC 3.6.3.14
• epididymis secretory sperm binding protein Li 123m
• hATP1
• HEL-S-123m
• MC5DN4
• MC5DN4A
• MC5DN4B
• mitochondrial ATP synthetase, oligomycin-resistant
• MOM2
• OMR
• ORM
• ORMATP synthase alpha chain, mitochondrial

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for ATP5A Full Length Recombinant Protein (H00000498-P01). (Showing 1 - 1 of 1 FAQ).

1. We are interested in custom synthesis of the same protein where one or both of the two cysteines would be mutated. Do you do custom work?
   • Unfortunately for product H00000498-P01, we are just the distributor for this item. The protein is manufactured by a Taiwanese company called Abnova. We are only able to provide their current products in the form present on our datasheets.

Bioinformatics

• Gene Symbol: ATP5F1A