ATP Citrate Lyase Antibody - BSA Free

Western Blot: ATP Citrate Lyase Antibody [NBP2-15510] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP Citrate Lyase Antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody [NBP2-15510] - ATP Citrate Lyase Antibody detects ATP Citrate Lyase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATP Citrate Lyase protein stained by ATP Citrate Lyase Antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP2-15510] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Western Blot: ATP Citrate Lyase Antibody [NBP2-15510] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP Citrate Lyase antibody [N1N2], N-term (NBP2-15510) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP2-15510] - ATP Citrate Lyase antibody [N1N2], N-term detects ATP Citrate Lyase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.ATP Citrate Lyase stained by ATP Citrate Lyase antibody [N1N2], N-term (NBP2-15510) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP Citrate Lyase Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ATP Citrate Lyase. The exact sequence is proprietary.
• Localization: Cytoplasm;
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACLY
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 121 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ATP Citrate Lyase Antibody - BSA Free

• ACLEC 2.3.3.8
• ACLY
• ATP Citrate Lyase
• ATP citrate synthase
• ATP-citrate (pro-S-)-lyase
• ATP-citrate synthase
• ATPCL
• Citrate cleavage enzyme
• CLATP
• EC 2.3.3.8

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ACLY
• Uniprot:
  • P53396(Human)