ATP Citrate Lyase Antibody - BSA Free

Western Blot: ATP Citrate Lyase Antibody [NBP1-33430] - Sample (30 ug of whole cell lysate) A: Hela B: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody [NBP1-33430] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [C2C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.

Western Blot: ATP Citrate Lyase Antibody [NBP1-33430] - Sample (50 ug of whole cell lysate) A: mouse brain 5% SDS PAGE, antibody diluted at 1:2000.

Western Blot: ATP Citrate Lyase Antibody [NBP1-33430] - A. 30 ug PC-12 whole cell lysate/extract, B. 30 ug Rat 2 whole cell lysate/extract 5% SDS-PAGE gel, antibody dilution 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP Citrate Lyase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATP Citrate Lyase Antibody - BSA Free (NBP1-33430) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ATP Citrate Lyase. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACLY
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 121 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ATP Citrate Lyase Antibody - BSA Free

• ACLEC 2.3.3.8
• ACLY
• ATP Citrate Lyase
• ATP citrate synthase
• ATP-citrate (pro-S-)-lyase
• ATP-citrate synthase
• ATPCL
• Citrate cleavage enzyme
• CLATP
• EC 2.3.3.8

Background

ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ACLY