ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free

Western Blot: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP Citrate Lyase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP Citrate Lyase.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded lung tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded bladder tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded colon tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded prostate tissue using anti-ATP Citrate Lyase mouse monoclonal antibody.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded Carcinoma of liver tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ACLY mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-ACLY mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded endometrium tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded Kidney tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded liver tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50.

Immunohistochemistry: ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free [NBP2-70073] - Staining of paraffin-embedded pancreas tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI3G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free (NBP2-02410) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ACLY (NP_001087) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACLY
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 120.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ATP Citrate Lyase Antibody (OTI3G8) - Azide and BSA Free

• ACLEC 2.3.3.8
• ACLY
• ATP Citrate Lyase
• ATP citrate synthase
• ATP-citrate (pro-S-)-lyase
• ATP-citrate synthase
• ATPCL
• Citrate cleavage enzyme
• CLATP
• EC 2.3.3.8

Background

ATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACLY