ATIC Antibody

Western Blot: ATIC Antibody [NBP1-32221] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE ATIC antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ATIC Antibody [NBP1-32221] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: ATIC Antibody [NBP1-32221] - Paraffin-embedded Colon ca, using antibody at 1:100 dilution.

Product Discontinued

ATIC Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATIC. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATIC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ATIC Antibody

• 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMPcyclohydrolase
• 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotidetransformylase/inosinicase
• AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme
• AICAR
• AICARFT
• AICARFT/IMPCHASE
• bifunctional purine biosynthesis protein PURH
• IMPCHASE
• phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
• PURHFLJ93545

Background

ATIC is a 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase which helps catalyze the penultimate and final steps of the de novo purine biosynthetic pathway. The human ATIC cDNA encodes a deduced 591 amino acid protein that is 81% identical to the chicken sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ATIC