ATG4A Antibody (514914) [Janelia Fluor® 525]

• Reactivity: Hu, Mu
• Applications: WB, IP, ICC/IF
• Clone: 514914
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

ATG4A Antibody (514914) [Janelia Fluor® 525] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4A isoform 1
  Glu2-Val398
  Accession # Q8WYN0
• Specificity: Detects human ATG4A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human ATG4B is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATG4A
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for ATG4A Antibody (514914) [Janelia Fluor® 525]

• APG4 autophagy 4 homolog A (S. cerevisiae)
• Apg4a
• ATG4 autophagy related 4 homolog A (S. cerevisiae)
• ATG4A
• Atg4al
• Autl2
• AUT-like 2 cysteine endopeptidase
• AUT-like 2, cysteine endopeptidase (S. cerevisiae)
• Autophagin 2
• autophagin-2
• cysteine endopeptidase
• cysteine protease ATG4A
• EC 3.4.22
• EC 3.4.22.-
• hAPG4A

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified.; FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.; ENZYME REGULATION: Inhibited by N-ethylmaleimide; SUBCELLULAR LOCATION: Cytoplasm;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG4A