ATF6 Antibody - BSA Free

Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.

Simple Western: ATF6 Antibody [NBP1-75478] - Simple Western lane view shows a specific band for ATF6 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-ATF6 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: ATF6 Antibody - BSA Free [NBP1-75478] - AICAR inhibits ER stress responses. Western analysis of cell lysates of nuclear extracts. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27562249/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - Antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ATF6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATF6 Antibody - BSA Free (NBP1-75478) is a polyclonal antibody validated for use in WB, ICC/IF and Simple Western. Anti-ATF6 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A partial recombinant protein made to an internal region of the human ATF6 protein (within residues 200-350). [Swiss-Prot P18850]
• Localization: Endoplasmic reticulum membrane, nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Simple Western 1:25
  • Western Blot 1:100-1:2000
• Application Notes: This ATF6 antibody is useful for Immunocytochemistry/Immunofluorescence.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:25, apparent MW was 101 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  HeLa DTT Treated / Untreated Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31235574).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for ATF6 Antibody - BSA Free

• ACHM7
• Activating transcription factor 6 alpha
• activating transcription factor 6
• atf6 a
• ATF6 alpha
• ATF6
• ATF6A
• ATF6-alpha
• cAMP-dependent transcription factor ATF-6 alpha
• cyclic AMP-dependent transcription factor ATF-6 alpha

Background

Endoplasmic reticulum stress results in protein folding irregularities and proteolytic abberations. ER stress is mediated in part by the activating transcription factor (ATF6). ATF6 is a signal transducer that prohibits phosphorylation of certain proteins, like the insulin receptor, and protects overactive responses to ER stress. This ultimately mediates apoptosis through mitochondrial cellular signaling. Influenza infections are known to affect ER stress in lung endothelial cells, partially mediated by ATF6 activity. This protein is also being investigated for potential optimization of recovery efforts, following traumatic injury, especially in spinal cord and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATF6 Antibody (NBP1-75478)(6)

Publications using NBP1-75478

• Xu F, Wang L., et Al. Deciphering ER stress-unfolded protein response relationship by visualizing unfolded proteins in the ER Cell Rep 2024-07-01 [PMID: 38865243]
• Thu Nguyen Minh Pham, Natarajan Perumal, Caroline Manicam, Marion Basoglu, Stefan Eimer, Dominik C. Fuhrmann, Claus U. Pietrzik, Albrecht M. Clement, Hagen Körschgen, Jana Schepers, Christian Behl Adaptive responses of neuronal cells to chronic endoplasmic reticulum (ER) stress Redox Biology 2023-10-20 [PMID: 37883843]
• Li T, Zhao H, Guo G et al. VMP1 affects endoplasmic reticulum stress sensitivity via differential modulation of the three unfolded protein response arms Cell reports 2023-03-28 [PMID: 36870060] (Western Blot, Human)
• Tabbarah S, Tavares E, Charish J et al. COG5 variants lead to complex early onset retinal degeneration, upregulation of PERK and DNA damage Scientific reports 2020-12-04 [PMID: 33277529] (WB, Human, Mouse)
• Park SJ, Kim Y, Yang SM et al. Discovery of endoplasmic reticulum calcium stabilizers to rescue ER-stressed podocytes in nephrotic syndrome Proc. Natl. Acad. Sci. U.S.A. 2019-06-24 [PMID: 31235574] (WB, Mouse)
• Bob M, Newbatt Y, Gupta S et al. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Sci Rep. 2016-08-26 [PMID: 27562249] (WB, Human)

Review for ATF6 Antibody (NBP1-75478) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/31/2020

Summary

• Application: Western Blot
• Sample Tested: Human primary fibroblast
• Species: Human
• Lot: b-1

Comments

• Comments: TGX 12% gel.10 ug of protein loaded reduced with DTT. Primary antibody incubation O/N 4ºC 1:1000 in BSA 3% PBST (0.1% Tween). Secondary ab: Donkey anti-rabit Alexa Fluor 647 1:5000 1h RT in BSA 3% PBST (0.1% Tween). ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

FAQs for ATF6 Antibody (NBP1-75478). (Showing 1 - 2 of 2 FAQs).

1. We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
   • The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.
2. Will this antibody detect the cleaved portion of ATF6 (60-80kDa), as well as the non-cleaved portion (110 kDa)? We are using human lymphocyte cell lysates (samples of patients with leukemia) on a proteinsimple Wes machine.
   • Thank you for contacting Novus regarding the ATF6 Antibody (NBP1-75478). We do see the full length in Simple Western but not the cleavage product. Nevertheless we assume you should see the 46kD cleavage product since the immunogen is located there but only under ER stress. The actual epitope is not mapped but the immunogen is with in the region of a.a. 200-250. Since the cleavage site is at 419-420 then we assume it is possible to detect this portion of the cleavge product but not the 27KD product without the immunogen sequence. 74 kD full length        aa1-420=46kD       aa420-670=27kD

Bioinformatics

• Gene Symbol: ATF6
• Entrez:
  • 22926(Human)
• Uniprot:
  • P18850()