ATF3 Antibody (CL1685) - Azide and BSA Free

Analysis in human placenta and tonsil tissues using NBP3-43991 antibody. Corresponding ATF3 RNA-seq data are presented for the same tissues.

Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.

Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Staining of RH-30 cells using the Anti-ATF3 monoclonal antibody) .

Staining of human tonsil shows no positivity in lymphoid cells as expected.

Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL1685
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ATF3 Antibody (CL1685) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ATF3 Antibody (CL1685) - Azide and BSA Free (NBP2-34489) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P18847, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATF3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ATF3 Antibody (CL1685) - Azide and BSA Free

• activating transcription factor 3cAMP-dependent transcription factor ATF-3
• ATF3deltaZip2
• ATF3deltaZip2c
• ATF3deltaZip3
• cyclic AMP-dependent transcription factor ATF-3

Background

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF3