Astrin Antibody


Immunocytochemistry/ Immunofluorescence: Astrin Antibody [NB100-74638] - CENP-E Is Required for Tethering Specifically to Lateral Walls of Microtubules. Immunofluorescence images of control or CENP-E siRNA-transfected more
Independent Antibodies: Immunoprecipitation: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of Immunoprecipitants. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from more

Product Details

Reactivity HuSpecies Glossary
Applications IB, ICC/IF, IP, WB (-)
1.0 mg/ml
Validated by:

Independent Antibodies


Order Details

Astrin Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human astrin (mitotic spindle associated protein p126) using the numbering given in entry NP_006452.3 (GeneID 10615).
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Not recommended for western blot. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23891108). Use in Immunoblotting reported in scientific literature (PMID:31808746).
Astrin Overexpression Lysate
Astrin Knockout 293T Cell Lysate
Read Publications using
NB100-74638 in the following applications:

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Astrin Antibody

  • Astrin
  • hMAP126
  • MAP126mitotic spindle coiled-coil related protein
  • mitotic spindle associated protein p126
  • Mitotic spindle-associated protein p126
  • sperm associated antigen 5
  • sperm-associated antigen 5


This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Ce
Applications: WB, ELISA, ICC/IF, IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po
Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Ec, All-NA
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P, KD
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P

Publications for Astrin Antibody (NB100-74638)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IB, ICC/IF.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-74638 Applications Species
Shrestha RL, Conti D, Tamura N et al. Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells Nat Commun 2017 Jul 28 [PMID: 28751710] (ICC/IF, Human) ICC/IF Human
Shrestha RL, Draviam VM et al. Lateral to End-on Conversion of Chromosome-Microtubule Attachment Requires Kinesins CENP-E and MCAK. Curr Biol 2013 Jul 23 [PMID: 23891108] (ICC/IF, Human) ICC/IF Human
Scott SJ, Li X, Jammula S et al. Evidence that polyploidy in esophageal adenocarcinoma originates from mitotic slippage caused by defective chromosome attachments bioRxiv Jan 1 2020 (ICC/IF, Human) ICC/IF Human
Gama Braga L The function of the pseudokinase domain of BUBR1 in mitosis. Thesis Jan 1 2020
Conti D, Gul P, Islam A, et al. Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation Elife Dec 6 2019 [PMID: 31808746] (IB, ICC/IF, Human) IB, ICC/IF Human
Elowe S, Braga LG, Landry C et al. The BUBR1 pseudokinase domain promotes efficient kinetochore PP2A-B56 recruitment to regulate spindle checkpoint silencing and chromosome alignment bioRxiv Aug 13 2019 (ICC/IF, Human) ICC/IF Human

Reviews for Astrin Antibody (NB100-74638) (0)

There are no reviews for Astrin Antibody (NB100-74638). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Astrin Antibody (NB100-74638). (Showing 1 - 1 of 1 FAQs).

  1. We recently bought Astrin antibody from your company (catalog # NB100-74638, date of purchase 24/01/2012). We did not realize this antibody is not for Western blotting until we received it, and we failed to optimize the antibody detection. Because the other Astrin antibodies are still available from Novus Biologicals, we are thinking to purchase another Astrin antibody. However, in order to ensure the successful use of antibody for our research, we would like to ask which Astrin antibody is guaranteed for Western blotting and possibly for immunofluorescence using human cells ? Also, in the case if the antibody does not work, would you consider the refunding or sending us the alternative antibody?
    • We have 6 antibodies that match your requirements, and they can be viewed on our website by searching for Astrin and narrowing your results using the filters on the left-hand side of the screen. We offer a 100% guarantee for the species and applications listed on the products' datasheets and are happy to provide refunds or replacements in the event that they do not perform as promised!

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional Astrin Products

Bioinformatics Tool for Astrin Antibody (NB100-74638)

Discover related pathways, diseases and genes to Astrin Antibody (NB100-74638). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Astrin Antibody (NB100-74638)

Discover more about diseases related to Astrin Antibody (NB100-74638).

Pathways for Astrin Antibody (NB100-74638)

View related products by pathway.

PTMs for Astrin Antibody (NB100-74638)

Learn more about PTMs related to Astrin Antibody (NB100-74638).

Research Areas for Astrin Antibody (NB100-74638)

Find related products by research area.

Blogs on Astrin

There are no specific blogs for Astrin, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Astrin Antibody and receive a gift card or discount.


Gene Symbol SPAG5