Astrin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with ...read more
Independent Antibodies: Immunoprecipitation: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of Immunoprecipitants. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

Astrin Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human astrin (mitotic spindle associated protein p126) using the numbering given in entry NP_006452.3 (GeneID 10615).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPAG5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Not recommended for western blot. Use in ICC/IF reported in scientific literature (PMID: 23891108).
Control
Astrin Overexpression Lysate
Publications
Read Publications using
NB100-74638 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Astrin Antibody

  • Astrin
  • DEEPEST
  • hMAP126
  • MAP126mitotic spindle coiled-coil related protein
  • mitotic spindle associated protein p126
  • Mitotic spindle-associated protein p126
  • sperm associated antigen 5
  • sperm-associated antigen 5

Background

This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Astrin Antibody (NB100-74638)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(5)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-74638 Applications Species
Song X, Conti D, Shrestha RL Et al. Counteraction between Astrin-PP1 and Cyclin-B-CDK1 pathways protects chromosome-microtubule attachments independent of biorientation Nature communications Dec 1 2021 [PMID: 34853300] (ICC/IF, Human) ICC/IF Human
Scott SJ, Li X, Jammula S et al. Evidence that polyploidy in esophageal adenocarcinoma originates from mitotic slippage caused by defective chromosome attachments Cell Death Differ Mar 2 2021 [PMID: 33649470]
Shrestha RL, Conti D, Tamura N et al. Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells Nat Commun 2017 Jul 28 [PMID: 28751710] (ICC/IF, Human) ICC/IF Human
Shrestha RL, Draviam VM et al. Lateral to End-on Conversion of Chromosome-Microtubule Attachment Requires Kinesins CENP-E and MCAK. Curr Biol 2013 Jul 23 [PMID: 23891108] (ICC/IF, Human) ICC/IF Human
Gama Braga L The function of the pseudokinase domain of BUBR1 in mitosis. Thesis Jan 1 2020
Shrestha RL, Rossi A, Wangsa D et al. CENP-A overexpression promotes aneuploidy with karyotypic heterogeneity The Journal of cell biology Apr 5 2021 [PMID: 33620383]
Gama Braga L, Cisneros AF, Mathieu MM et al. BUBR1 Pseudokinase Domain Promotes Kinetochore PP2A-B56 Recruitment, Spindle Checkpoint Silencing, and Chromosome Alignment Cell Rep Nov 17 2020 [PMID: 33207204] (ICC/IF, Human)

Details:
HeLa T-Rex and HeLa S3 cell lines
ICC/IF Human
Conti D, Gul P, Islam A, et al. Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation Elife 2019 Dec 6 [PMID: 31808746] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Astrin Antibody (NB100-74638). (Showing 1 - 1 of 1 FAQs).

  1. We recently bought Astrin antibody from your company (catalog # NB100-74638, date of purchase 24/01/2012). We did not realize this antibody is not for Western blotting until we received it, and we failed to optimize the antibody detection. Because the other Astrin antibodies are still available from Novus Biologicals, we are thinking to purchase another Astrin antibody. However, in order to ensure the successful use of antibody for our research, we would like to ask which Astrin antibody is guaranteed for Western blotting and possibly for immunofluorescence using human cells ? Also, in the case if the antibody does not work, would you consider the refunding or sending us the alternative antibody?
    • We have 6 antibodies that match your requirements, and they can be viewed on our website by searching for Astrin and narrowing your results using the filters on the left-hand side of the screen. We offer a 100% guarantee for the species and applications listed on the products' datasheets and are happy to provide refunds or replacements in the event that they do not perform as promised!

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPAG5
Entrez
Uniprot