Astrin Antibodies
We offer Astrin Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each Astrin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Astrin Antibodies can be used in a variety of model species: Human. Use the list below to choose the Astrin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Astrin polyclonal antibodies and browse our Astrin monoclonal antibody catalog.
Alternate Names for Astrin Antibodies
anti-Astrin antibody, anti-SPAG5 antibody, anti-DEEPEST antibody, anti-hMAP126 antibody, anti-MAP126mitotic spindle coiled-coil related protein antibody, anti-mitotic spindle associated protein p126 antibody, anti-Mitotic spindle-associated protein p126 antibody, anti-sperm associated antigen 5 antibody, anti-sperm-associated antigen 5 antibody
9 Results for "astrin" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with antibodies against Astrin and tubulin and CREST antisera and stained with DAPI for DNA. Cropped images are 3x magnified showing lateral kinetochores. Scale bars represent 5 um (insets, 2 um). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0960982213007653), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74638-img0003.jpg "Immunocytochemistry/Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with antibodies against Astrin and tubulin and CREST antisera and stained with DAPI for DNA. Cropped images are 3x magnified showing lateral kinetochores. Scale bars represent 5 um (insets, 2 um). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0960982213007653), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of Immunoprecipitants. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74638 used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74639, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunoprecipitation-NB100-74638-img0002.jpg)
![Immunohistochemistry: Astrin Antibody [NB100-74638] - SKA3 localisation is not abrogated due to the lack of Astrin.(A) Representative deconvolved images show SKA3 enrichment at kinetochores of HeLa cells treated with Control or Astrin siRNA. Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against Astrin & SKA3 & CREST antiserum (to mark centromeres) & stained with DAPI for DNA. Cropped images show Astrin or SKA3 enrichment at kinetochores. (B) Representative images show SKA3 enrichment at kinetochores of HeLa cells treated with Astrin siRNA before transfection with eukaryotic protein expression vectors encoding YFP-Astrin (WT, 4A or ∆70, as indicated). Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against GFP (for detecting YFP-Astrin) & SKA3 & CREST antiserum & co-stained with DAPI for DNA. Cropped images show YFP-Astrin or SKA3 enrichment at kinetochores. Scale as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31808746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74638_rabbit-polyclonal-astrin-antibody-31020241612667.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human testis and endometrium tissues using NBP2-88942 antibody. Corresponding SPAG5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human testis and endometrium tissues using NBP2-88942 antibody. Corresponding SPAG5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Western-Blot-NBP2-88942-img0006.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human endometrium shows very weak cytoplasmic positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0001.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human prostate shows no cytoplasmic positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0002.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0003.jpg)
![Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Astrin-Antibody-CL10078-Immunohistochemistry-Paraffin-NBP2-88942-img0004.jpg)
![Immunoprecipitation: Astrin Antibody - BSA Free [NBP3-45856] - IP Result of anti-Astrin (IP:, 5ug; Detection: 1:1000) with HeLa cells lysate 3000ug.](http://images.novusbio.com/fullsize/nbp3-45856_rabbit-astrin-pab-bsa-free-284202512365487.jpg "Immunoprecipitation: Astrin Antibody - BSA Free [NBP3-45856] - IP Result of anti-Astrin (IP:, 5ug; Detection: 1:1000) with HeLa cells lysate 3000ug.")
![Immunohistochemistry: Astrin Antibody - BSA Free [NBP3-45856] - Immunohistochemistry of paraffin-embedded human testis using (Astrin antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-45856_rabbit-astrin-pab-bsa-free-28420251304157.jpg)
![Western Blot: Astrin Antibody - BSA Free [NBP3-45856] - HeLa cells were subjected to SDS PAGE followed by western blot with (Astrin antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-45856_rabbit-astrin-pab-bsa-free-284202512365472.jpg)
![Western Blot: Astrin Antibody [NBP1-85262] - Analysis in control (vector only transfected HEK293T lysate) and SPAG5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Astrin-Antibody-Western-Blot-NBP1-85262-img0007.jpg "Western Blot: Astrin Antibody [NBP1-85262] - Analysis in control (vector only transfected HEK293T lysate) and SPAG5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Astrin Antibody [NBP1-85262] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunohistochemistry-Paraffin-NBP1-85262-img0006.jpg)
![Western Blot: Astrin Antibody [NB100-74639] - Detection of Human Astrin/MAP126 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74639 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74638 , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/Astrin-Antibody-Western-Blot-NB100-74639-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Astrin Antibody [NB100-74639] - Detection of Human Astrin/MAP126 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74639 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74638 , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![Immunocytochemistry/Immunofluorescence: Astrin Antibody [NBP3-17806] - Staining of human cell line HAP1 shows localization to nuclear bodies, cytosol & mitotic spindle.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17806-img0001.jpg "Immunocytochemistry/Immunofluorescence: Astrin Antibody [NBP3-17806] - Staining of human cell line HAP1 shows localization to nuclear bodies, cytosol & mitotic spindle.")