Astrin Antibody (CL10078) - BSA Free

Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human testis and endometrium tissues using NBP2-88942 antibody. Corresponding SPAG5 RNA-seq data are presented for the same tissues.

Western Blot: Astrin Antibody (CL10078) [NBP2-88942] - Analysis in human cell line RH-30.

Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human endometrium shows very weak cytoplasmic positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human prostate shows no cytoplasmic positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Astrin Antibody (CL10078) [NBP2-88942] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL10078
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Astrin Antibody (CL10078) - BSA Free Summary

• Description: Novus Biologicals Mouse Astrin Antibody (CL10078) - BSA Free (NBP2-88942) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from Q96R06, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPAG5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Astrin Antibody (CL10078) - BSA Free

• Astrin
• DEEPEST
• hMAP126
• MAP126mitotic spindle coiled-coil related protein
• mitotic spindle associated protein p126
• Mitotic spindle-associated protein p126
• sperm associated antigen 5
• sperm-associated antigen 5

Background

Astrin encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPAG5