Asialoglycoprotein Receptor 2 Antibody (002) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Asialoglycoprotein Receptor 2 Antibody (002) [NBP3-06211] - Immunofluorescence staining of ASGR2 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ASGR2 monoclonal antibody (1:60) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody (002) [NBP3-06211] - Staining of human ASGR2 in human liver with rabbit monoclonal antibody (1:200).

Flow Cytometry: Asialoglycoprotein Receptor 2 Antibody (002) [NBP3-06211] - Flow cytometric analysis of Human ASGR2 expression on HepG2 cells. Cells were stained with purified anti-Human ASGR2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody (002) [NBP3-06211] - Staining of human ASGR2 in human cirrhosis with rabbit monoclonal antibody (1:200).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC
• Clone: 002
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Asialoglycoprotein Receptor 2 Antibody (002) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human Asialoglycoprotein Receptor 2 (Uniprot#: P07307-1; Gln80-Ala311).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ASGR2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunohistochemistry-Paraffin 1:100-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Asialoglycoprotein Receptor 2 Antibody (002) - Azide and BSA Free

• ASGPR 2
• ASGP-R 2
• ASGPR2
• ASGP-R2
• ASGR2
• asialoglycoprotein receptor 2
• CLEC4H2
• CLEC4H2FLJ60040
• C-type lectin domain family 4 member H2
• HBxAg-binding protein
• HBXBP
• Hepatic lectin H2
• HL-2

Background

Asialoglycoprotein Receptor 2 is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of plasma glycoproteins in which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. It is expressed exclusively in hepatic parenchymal cells and calcium is required for ligand binding. Asialoglycoprotein Receptor 2 may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. Asialoglycoprotein Receptor 2 is involved in hepatitis B, hepatitis C, liver cirrhosis, hepatocellular carcinoma, nephropathy, leukemia, urethritis, autoimmune hepatitis, alcoholic liver cirrhosis, and jaundice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASGR2