ASCL1/Mash1 Antibody (1C5)

Images

 
Western Blot: ASCL1/Mash1 Antibody (1C5) [H00000429-M04] - Analysis of ASCL1 expression in A-549 (Cat # L025V1).
ELISA: ASCL1/Mash1 Antibody (1C5) [H00000429-M04] - Detection limit for recombinant GST tagged ASCL1 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ASCL1/Mash1 Antibody (1C5) Summary

Immunogen
ASCL1 (NP_004307, 137 a.a. - 236 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTNWF
Specificity
ASCL1 - achaete-scute complex-like 1 (Drosophila)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ASCL1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Positive Control
Brain Cerebral Cortex Lysate (NB820-59182)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ASCL1/Mash1 Antibody (1C5)

  • achaete-scute complex (Drosophila) homolog-like 1
  • achaete-scute complex homolog 1 (Drosophila)
  • achaete-scute complex-like 1 (Drosophila)
  • achaete-scute homolog 1
  • ASCL1
  • ASH1
  • ASH-1
  • ASH1MASH1
  • BHLHA46
  • bHLHa46achaete-scute complex-like 1
  • Class A basic helix-loop-helix protein 46
  • HASH1
  • HASH1achaete scute protein
  • Mash1

Background

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ASCL1
Entrez
OMIM
Uniprot