BVES Antibody [Unconjugated]

BVES was detected in immersion fixed paraffin-embedded sections of Human Heart using Sheep Anti-Human BVES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4986) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

BVES Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human BVES
  Met1-Asn36
  Accession # Q8NE79
• Specificity: Detects human BVES in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: BVES
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BVES Antibody [Unconjugated]

• blood vessel epicardial substance
• BVES
• H
• HBVES
• POP1
• POP1MGC42413
• POPDC1
• POPDC1popeye domain containing 1
• Popeye domain-containing protein 1
• Popeye protein 1

Background

BVES (blood vessel epicardial substance; also Popeye 1) is a 58 kDa, glycoprotein member of the Popeye family of multispan transmembrane (TM) molecules. It is expressed on embryonic epicardial epithelium plus atrial myocytes and on adult skeletal and cardiac muscle. It serves as a component of the epithelial tight junction, dimerizing with itself and interacting with ZO-1. Human BVES is 360 amino acids (aa) in length. It contains an extracellular N-terminus of 48 aa, three TM domains (aa 49‑113) and a large C-terminal cytoplasmic region (aa 114‑360) that contains a Popeye domain (aa 123‑266). Homodimerization occurs via aa 268‑274. There is one potential splice variant that shows a premature truncation after Glu213. Over aa 1‑36, human BVES is 72% aa identical to mouse BVES.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BVES
• Uniprot:
  • Q8NE79()