ASC/TMS1 Antibody

Western Blot: ASC/TMS1 Antibody [NB100-56365] - Analysis of extracts from HL60 cells using ASC/TMS1 antibody (NB100-56365) at 1:1000 (0.5ug/ml).

Western Blot: ASC/TMS1 Antibody [NB100-56365] - Analysis for ASC/TMS1 using NB100-56365 at 2 ug/ml against 10 ug/lane of A549 cell lysate.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ASC/TMS1 Antibody Summary

• Immunogen: This polyclonal antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 31-46 and 94-110 of human TMS1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PYCARD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Control:

  ASC/TMS1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2% Gelatin
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for ASC/TMS1 Antibody

• ASC
• ASCMGC10332
• CARD5
• CARD5TMS
• caspase recruitment domain protein 5
• Caspase recruitment domain-containing protein 5
• hASC
• PYCARD
• PYD and CARD domain containing
• PYD and CARD domain-containing protein
• Target of methylation-induced silencing 1
• TMS1
• TMS-1
• TMS1apoptosis-associated speck-like protein containing a CARD

Background

TMS1 (target of methylation-induced silencing) is a recently identified CpG island-associated gene that becomes hypermethylated and silenced in cells overexpressing DNA cytosine-5-methyltransferase-1 (1). TMS1 is aberrantly methylated and silenced in human breast cancer cells. Forty percent (11 of 27) of primary breast tumors exhibited aberrant methylation of TMS1. Ectopic expression of TMS1 induced apoptosis in 293 cells and inhibited the survival of human breast cancer cells. The methylation-mediated silencing of TMS1 may confer a survival advantage by allowing cells to escape from apoptosis, supporting a new role for aberrant methylation in breast tumorigenesis. TMS1 encodes a 22-kDa predicted protein containing a COOH-terminal caspase recruitment domain (CARD). However, it is structurally unrelated to other known CARD adaptor and regulatory proteins. Ectopic expression of TMS1 alone was able to trigger apoptosis in 293 cells, and cell death correlated with relocalization of TMS1 from the cytoplasm to perinuclear, ball-like structures. Several lines of evidence support the idea that redistribution of TMS1 is an intermediate step in a TMS1-triggered apoptotic pathway (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASC/TMS1 Antibody (NB100-56365)(1)

Publication using NB100-56365

• Siraj AK, Hussain AR, Al-Rasheed M et al. Demethylation of TMS1 gene sensitizes thyroid cancer cells to TRAIL-induced apoptosis. J Clin Endocrinol Metab. 2011 Jan [PMID: 20926535] (WB)
  
  Details:
  WB: TMS1 (IMG-426). 1. Human thyroid cancer cell line; B-CPAP1, ML-1, WR082-1, CAL-62, BHT-101, 8505C, TT2609-C02, Fig 1C.

Bioinformatics

• Gene Symbol: PYCARD
• Entrez:
  • 29108(Human)
• Uniprot:
  • Q9ULZ3()