ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody

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Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP1-76360] - ASAH2 Antibody 293 cell lysate with ASAHL antibody at (A) 1 and (B) 2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2. The immunogen is located within the last 50 amino acids of ASAH2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASAH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
Control Peptide
ASAH2/N-acylsphingosine Amidohydrolase-2 Peptide (NBP1-76360PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-76360 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody

  • ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
  • ASAH2
  • BCDase
  • HNAC1
  • LCDase
  • Nacylsphingosine Amidohydrolase2
  • NCDase
  • N-CDase

Background

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody (NBP1-76360) (0)

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Review for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody (NBP1-76360) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-76360:
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(1)
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Images Ratings Applications Species Date Details
Western Blot ASAH2/N-acylsphingosine Amidohydrolase-2 NBP1-76360
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reviewed by:
Anonymous
WB Human 12/18/2017
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Summary

ApplicationWestern Blot
Sample Testedhuman primary hepatocytes
SpeciesHuman
Lot4743-0803

Comments

CommentsName: Anti-ASAH2 antibody (NBP1-76360)
Catalog #: Anti-ASAH2 antibody (NBP1-76360)
Lot Number: Anti-ASAH2 antibody (NBP1-76360, Lot # 4743-0803)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): lane 1: human primary hepatocytes; lane 2: Hep G2
Sample Information:
     Cell Line or Tissue: human primary hepatocytes; and Hep G2
     Species: human
     Treatment: No treatment
Lysate Preparation:
     Date of lysate preparation: December 4, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: No
     Negative Control: No
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 10%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/1000
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times
Secondary Antibody
Manufacturer and Catalog #: Promega, W401B, Lot # 0000187662
Secondary description: goat anti-rabbit secondary antibody
     Dilution: 1/2000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature

Detection Method:
Detection: ECL (GE, cat # RPN2209, lot # 9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 45 seconds
Molecular weight of band(s): ~ 70 kDa
Experimental Concerns and Observations: A specific band around 70 kDa was observed

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Recent Reviews

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4
1
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2
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1
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Anonymous
12/18/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol ASAH2
Uniprot