ASAH3 Antibody - BSA Free

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Western Blot: ASAH3 Antibody [NBP1-74083] - HCT15 Cell Lysate 1ug/ml Gel Concentration 12%

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

ASAH3 Antibody - BSA Free Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to the C terminal of ACER1. Immunizing peptide sequence VNAYALNSIALHILYIVCQEYRKTSNKELRHLIEVSVVLWAVALTSWISD. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACER1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for ASAH3 Antibody - BSA Free

  • Acylsphingosine deacylase 3
  • Alkaline CDase 1
  • alkaline ceramidase 1
  • AlkCDase 1
  • ALKCDase1
  • ASAH3
  • EC 3.5.1.23
  • MGC138327
  • MGC138329
  • N-acylsphingosine amidohydrolase (alkaline ceramidase) 3
  • N-acylsphingosine amidohydrolase 3

Background

ACER1 hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. It has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone. It may have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ACER1
Uniprot