ARK5/NUAK1 Antibody

Immunocytochemistry/Immunofluorescence: ARK5/NUAK1 Antibody [NBP1-36937] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: ARK5/NUAK1 Antibody [NBP1-36937] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody

• Reactivity: Hu, Rt, Bv
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARK5/NUAK1 Antibody Summary

• Description: Novus Biologicals Goat ARK5/NUAK1 Antibody (NBP1-36937) is a polyclonal antibody validated for use in ELISA, Flow and ICC/IF. Anti-ARK5/NUAK1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QNRPRPQYLKRYRNR corresponding to internal region according to NP_055655.1.
• Epitope: internal region
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NUAK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:8000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARK5/NUAK1 Antibody

• AMPK-related protein kinase 5
• ARK5
• ARK5AMP-activated protein kinase family member 5
• EC 2.7.11
• EC 2.7.11.1
• KIAA0537NUAK family SNF1-like kinase 1
• NUAK family, SNF1-like kinase, 1
• NUAK1

Background

NUAK1 is involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARK5/NUAK1 Antibody (NBP1-36937)(1)

Publication using NBP1-36937

• Kusakai G, Suzuki A, Ogura T et al. Strong association of ARK5 with tumor invasion and metastasis. J Exp Clin Cancer Res 2004-06-01 [PMID: 15354411]

Bioinformatics

• Gene Symbol: NUAK1
• Entrez:
  • 9891(Human)
  • 299694(Rat)
• Uniprot:
  • NP_055655.1(Human)