ARID5A Antibody (1683) - Azide and BSA Free

Western Blot: ARID5A Antibody (1683) [NBP2-43676] - THP-1 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [1683]diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676] - Mouse urinary bladder. ARID5A stained by ARID5A antibody [1683] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: ARID5A Antibody (1683) [NBP2-43676] - Analysis of Non-transfected (-) and ARID5A -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:10000.

Immunohistochemistry-Frozen: ARID5A Antibody (1683) [NBP2-43676] - detects ARID5A protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse mouse cerebellum. Green: ARID5A stained by ARID5A antibody (1683) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676] - Mouse brain. ARID5A stained by ARID5A antibody [1683] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

ARID5A Antibody (1683) [NBP2-43676] - ARID5A Antibody (1683) detects ARID5A protein by flow cytometry analysis.Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: ARID5A Antibody (1683) dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC
• Clone: 1683
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ARID5A Antibody (1683) - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARID5A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for ARID5A Antibody (1683) - Azide and BSA Free

• ARID domain-containing protein 5A
• AT rich interactive domain 5A (MRF1-like)
• AT-rich interactive domain-containing protein 5A
• RFVG5814

Background

Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles indevelopment, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 (PubMed15640446)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARID5A