Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated]

Images

 
Melanotransferrin/CD228 was detected in perfusion fixed frozen sections of human melanoma tissue using Mouse Anti-Human Melanotransferrin/CD228 Monoclonal Antibody (Catalog # MAB8175) at 5 µg/mL for 1 hour at ...read more
Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Melanotransferrin/CD228 at approximately 109 kDa (as indicated) using 10 ...read more
Western blot shows lysates of SK‑Mel‑28 human malignant melanoma cell line and Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Melanotransferrin/CD228 Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
893438
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated] Summary

Immunogen
HEK293 human embryonic kidney cell line transfected with human Melanotransferrin/CD228
Met1-Gly711
Accession # P08582
Specificity
Detects human Melanotransferrin/CD228 in ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MFI2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated]

  • antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5
  • CD228 antigen
  • CD228
  • FLJ38863
  • MAP97
  • MAP97Melanoma-associated antigen p97
  • Melanotransferrin
  • membrane-bound transferrin-like protein
  • MFI2
  • MGC4856
  • MTF
  • MTF1
  • p97

Background

Melanotransferrin, also known as MTF, CD228, melanoma-associated antigen p97, MAP97 and MFI2, is a 90-97 kDa sialoglycoprotein member of the transferrin family. Unlike other transferrins, which are secreted, MTF is usually found tethered to the cell membrane by a glycosyl phosphatidyl inositol anchor, with only small amounts of soluble protein detected. MTF is highly expressed on melanoma cells, and at lower levels in salivary gland, pancreas, kidney and testis. Like other transferrins, MTF is an iron-binding protein, and may play roles in cellular proliferation, tumorigenesis, metastasis and migration. Full-length human, mouse and rat MTF is 738 amino acids (aa). Over aa 1-711, human MTF shares 85 and 86% aa identity with mouse and rat MTF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MFI2
Uniprot