ARHGEF5 Antibody

Images

 
Independent Antibodies: Western Blot: ARHGEF5 Antibody [NBP1-23000] - Whole cell lysate from HeLa cells. ARHGEF5 was also immunoprecipitated by rabbit anti-ARHGEF5 antibody NBP1-23001.
Immunocytochemistry/ Immunofluorescence: ARHGEF5 Antibody [NBP1-23000] - Human colon carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).
Immunohistochemistry: ARHGEF5 Antibody [NBP1-23000] - FFPE section of human colon carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

ARHGEF5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human Rho guanine nucleotide exchange factor (GEF) 5 using the numbering given in entry NP_005426.2 (GeneID 7984).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGEF5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
For IHC-P: Epitope retrieval with citrate buffer pH 6.0 is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARHGEF5 Antibody

  • ephexin-3
  • GEF5
  • Guanine nucleotide regulatory protein TIM
  • Oncogene TIM
  • p60 TIM
  • P60
  • Rho guanine nucleotide exchange factor (GEF) 5
  • rho guanine nucleotide exchange factor 5
  • TIM1
  • TIMDKFZp686N1969
  • Transforming immortalized mammary oncogene

Background

ARHGEF5, also known as TIM (transforming immortalized mammary), is an oncogene that belongs to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho GTPases. It is implicated to play a role in proliferative breast disease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARHGEF5
Entrez
Uniprot