ARHGEF5 Antibody

Western Blot: ARHGEF5 Antibody [NBP1-23000] - Whole cell lysate from HeLa cells. ARHGEF5 was also immunoprecipitated by rabbit anti-ARHGEF5 antibody NBP1-23001.

Immunohistochemistry-Paraffin: ARHGEF5 Antibody [NBP1-23000] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-APC7 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ARHGEF5 Antibody Summary

• Description: Novus Biologicals Rabbit ARHGEF5 Antibody (NBP1-23000) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human Rho guanine nucleotide exchange factor (GEF) 5 using the numbering given in entry NP_005426.2 (GeneID 7984).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARHGEF5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: For IHC-P: Epitope retrieval with citrate buffer pH 6.0 is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARHGEF5 Antibody

• ephexin-3
• GEF5
• Guanine nucleotide regulatory protein TIM
• Oncogene TIM
• p60 TIM
• P60
• Rho guanine nucleotide exchange factor (GEF) 5
• rho guanine nucleotide exchange factor 5
• TIM1
• TIMDKFZp686N1969
• Transforming immortalized mammary oncogene

Background

ARHGEF5, also known as TIM (transforming immortalized mammary), is an oncogene that belongs to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho GTPases. It is implicated to play a role in proliferative breast disease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARHGEF5
• Entrez:
  • 7984(Human)
• Uniprot:
  • Q12774(Human)