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ARHGEF4 Antibody (ARHGEF-08)

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Western Blot: ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217] - Analysis of ARHGEF4 in HEK293 cells (A) and HEK293-ARHGEF4 transfectants (B) using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08).
Flow (Intracellular): ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217] - Intracellular staining of ARHGEF4 in transfected HEK-293 cells using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08) PE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
ARHGEF-08
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ARHGEF4 Antibody (ARHGEF-08) Summary

Immunogen
Recombinant fragment of human ARHGEF4 (amino acids 143-271)
Specificity
The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ARHGEF4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular) 1-4 ug/ml
  • Flow Cytometry 1-4 ug/ml
  • Western Blot 1:100-1:2000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Purity
Protein A purified

Alternate Names for ARHGEF4 Antibody (ARHGEF-08)

  • APC-stimulated guanine nucleotide exchange factor 1
  • Asef
  • ASEF1
  • ASEFAPC-stimulated guanine nucleotide exchange factor
  • GEF4
  • KIAA1112
  • Rho guanine nucleotide exchange factor (GEF) 4
  • rho guanine nucleotide exchange factor 4
  • STM6

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through Gucleotide exchange protein coupled receptors. Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. This protein is similar to rat collybistin protein. Alternative splicing of the gene generates two transcript variants which encode different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARHGEF4