ARHGEF4 Antibody (ARHGEF-08) - BSA Free

Western Blot: ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217] - Analysis of ARHGEF4 in HEK293 cells (A) and HEK293-ARHGEF4 transfectants (B) using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08).

Flow (Intracellular): ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217] - Intracellular staining of ARHGEF4 in transfected HEK-293 cells using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08) PE.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: ARHGEF-08
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

ARHGEF4 Antibody (ARHGEF-08) - BSA Free Summary

• Description: Novus Biologicals Mouse ARHGEF4 Antibody (ARHGEF-08) - BSA Free (NBP2-62217) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fragment of human ARHGEF4 (amino acids 143-271)
• Specificity: The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARHGEF4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 1-4 ug/ml
  • Flow Cytometry 1-4 ug/ml
  • Western Blot 1:100-1:2000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: Protein A purified

Alternate Names for ARHGEF4 Antibody (ARHGEF-08) - BSA Free

• APC-stimulated guanine nucleotide exchange factor 1
• Asef
• ASEF1
• ASEFAPC-stimulated guanine nucleotide exchange factor
• GEF4
• KIAA1112
• Rho guanine nucleotide exchange factor (GEF) 4
• rho guanine nucleotide exchange factor 4
• STM6

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through Gucleotide exchange protein coupled receptors. Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. This protein is similar to rat collybistin protein. Alternative splicing of the gene generates two transcript variants which encode different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARHGEF4