ARH Antibody

Immunocytochemistry/Immunofluorescence: ARH Antibody [NB100-1276] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

Immunocytochemistry/Immunofluorescence: ARH Antibody [NB100-1276] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing showing cytoplasmic and some nuclear staining. T

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARH Antibody Summary

• Description: Novus Biologicals Goat ARH Antibody (NB100-1276) is a polyclonal antibody validated for use in ELISA and ICC/IF. Anti-ARH Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence DALKSAGRALIRS-C corresponding to N-Terminus according to NP_056442.2.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LDLRAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:16000
• Application Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells.
• Control:

  ARH Overexpression Lysate

• Control Peptide:

  ARH Peptide (NB100-1276PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARH Antibody

• ARH1
• ARH2
• ARHAutosomal recessive hypercholesterolemia protein
• DKFZp586D0624
• FHCB1ARH1
• FHCB2LDL receptor adaptor protein
• low density lipoprotein receptor adapter protein 1
• low density lipoprotein receptor adaptor protein 1
• MGC34705

Background

The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARH Antibody (NB100-1276)(2)

Publications using NB100-1276

• Ye Z-J, Go G-W, Singh R et al. LRP6 regulates LDLR-mediated LDL uptake. The Journal of Biological Chemistry. 2011-11-28 [PMID: 22128165]
• He G, Gupta S, Yi M et al. ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2. J Biol Chem 2002-11-15 [PMID: 12221107]

Bioinformatics

• Gene Symbol: LDLRAP1
• Entrez:
  • 26119(Human)
  • 100017(Mouse)
• Uniprot:
  • Q5SW96(Human)