Arginase 1/ARG1/liver Arginase Antibody (658922) [Phycoerythrin]

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Arginase 1/ARG1 PE-conjugated Mono-clonal Antibody (Catalog # IC8026P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin aM/CD11b APC-conjugated Monoclonal Antibody (Catalog # FAB1699A) and either (A) Mouse Anti-Human Arginase 1/ARG1 PE-conjugated Monoclonal Antibody (Catalog # IC8026P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 658922
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Arginase 1/ARG1/liver Arginase Antibody (658922) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human Arginase 1/ARG1
  Met1-Lys322
  Accession # P05089
• Specificity: Detects human Arginase 1/ARG1 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARG1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Arginase 1/ARG1/liver Arginase Antibody (658922) [Phycoerythrin]

• AI
• ARG1
• Arginase 1
• arginase, liver
• Arginase-1
• EC 3.5.3.1
• EC:3.5.3.1
• Liver Arginase
• Liver-type arginase
• PGIF
• Type I Arginase

Background

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human ARG1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is moderately active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arginase 1/ARG1/liver Arginase Antibody (IC8026P)(6)

Publications using IC8026P

• MM LaRue, S Parker, J Puccini, M Cammer, AC Kimmelman, D Bar-Sagi Metabolic reprogramming of tumor-associated macrophages by collagen turnover promotes fibrosis in pancreatic cancer Proceedings of the National Academy of Sciences of the United States of America, 2022-04-11;119(16):e2119168119. 2022-04-11 [PMID: 35412885] (Flow Cytometry, Mouse)
• T Kwak, F Wang, H Deng, T Condamine, V Kumar, M Perego, A Kossenkov, LJ Montaner, X Xu, W Xu, C Zheng, LM Schuchter, RK Amaravadi, TC Mitchell, GC Karakousis, C Mulligan, B Nam, G Masters, N Hockstein, J Bennett, Y Nefedova, DI Gabrilovic Distinct Populations of Immune-Suppressive Macrophages Differentiate from Monocytic Myeloid-Derived Suppressor Cells in Cancer Cell Reports, 2020-12-29;33(13):108571. 2020-12-29 [PMID: 33378668] (Flow Cytometry, Human)
• M Zarobkiewi, W Kowalska, S Chocholska, W Tomczak, A Szyma?ska, I Morawska, A Wojciechow, A Bojarska-J High M-MDSC Percentage as a Negative Prognostic Factor in Chronic Lymphocytic Leukaemia Cancers (Basel), 2020-09-14;12(9):. 2020-09-14 [PMID: 32937740] (ICC, Human)
• G Karina Chimal-Ram Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes J Immunol Res, 2016-06-08;2016(0):6031486. 2016-06-08 [PMID: 27376091] (Flow Cytometry, Human)
• NAICHUN ZHANG, JIANNING DENG, FENGYAO WU, XIANGCHAN LU, LEI HUANG, MIN ZHAO Expression of arginase I and inducible nitric oxide synthase in the peripheral blood and lymph nodes of HIV-positive patients Molecular Medicine Reports 2016-01-01 [PMID: 26647762]
• Lee S, Park H, Jeon S, Lee C, Seong R, Park S, Hong S Ubiquitous Over-Expression of Chromatin Remodeling Factor SRG3 Ameliorates the T Cell-Mediated Exacerbation of EAE by Modulating the Phenotypes of both Dendritic Cells and Macrophages. PLoS ONE, 2015-07-06;10(7):e0132329. 2015-07-06 [PMID: 26147219] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ARG1
• Uniprot:
  • P05089()