ARA54 Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

ARA54 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human ARA54
  Met127-Gln261
  Accession # Q9UBS8
• Specificity: Detects endogenous human ARA54 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARA54 Antibody [Alexa Fluor® 750]

• Androgen receptor-associated protein 54
• ARA54
• ARA54androgen receptor associated protein 54
• EC 6.3.2.-
• HFB30
• HFB30E3 ubiquitin-protein ligase RNF14
• ring finger protein 14FLJ26004
• RNF14
• Triad2 protein
• TRIAD2

Background

ARA54 (androgen receptor associated protein 54; also RING finger protein 14) is a 50‑55 kDa member of the zinc finger superfamily of proteins. It is ubiquitously expressed, and has two functions; one is to serve as a homodimeric co‑activator for androgen receptor‑induced transcription, and a second as an E3 ubiquitin-protein ligase. Human ARA54 is 474 amino acids (aa) in length and contains an RDW domain that interacts with E2 ubiquitin ligase (aa 11‑137), two zinc finger domains that qualify as RING-type (aa 220‑266) and IBR-type (aa 289‑350), and an androgen-interaction region (aa 361‑474) that contains a coiled‑coil domain. There is one alternate start site at Met127. Over aa 127‑261, human ARA54 is 87% aa identical to mouse ARA54.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.