APPL Antibody (709208) [Alexa Fluor® 647]

• Applications: WB
• Clone: 709208
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

APPL Antibody (709208) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human APPL
  Met1-Ile373
  Accession # Q9UKG1
• Specificity: Detects human APPL in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

APPL, also known as DCC interacting protein 13 alpha (DIP13 alpha), is a widely expressed approximately 80 kDa intracellular adaptor protein. APPL contains two coiled-coil domains (aa 215-259 and aa 621-673), a pleckstrin homology domain (aa 277-375) and a phosphotyrosine interaction domain (PID) (aa 496-656). These domains and the cytoplasmic and nuclear localization of APPL enable it to associate with a range of molecules involved in multiple pathways. APPL mediates the insulin-sensitizing and anti-inflammatory effects of Adiponectin through direct interactions with AdipoR1 and AdipoR2. Within aa 2-373, human APPL shares 99% aa sequence identity with mouse and rat APPL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.