APPBP1 Antibody (OTI1E10)

Western Blot: APPBP1 Antibody (OTI1E10) [NBP2-46087] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEDD8 Activating Enzyme.

Immunohistochemistry: APPBP1 Antibody (OTI1E10) [NBP2-46087] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: APPBP1 Antibody (OTI1E10) [NBP2-46087] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: APPBP1 Antibody (OTI1E10) [NBP2-46087] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

APPBP1 Antibody (OTI1E10) Summary

• Description: Novus Biologicals Mouse APPBP1 Antibody (OTI1E10) (NBP2-46087) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-274 of human APPBP1 (NP_001018170) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 50.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for APPBP1 Antibody (OTI1E10)

• A-116A10.1
• amyloid beta precursor protein binding protein 1, 59kDa
• Amyloid beta precursor protein-binding protein 1, 59 kDa
• amyloid beta precursor protein-binding protein 1, 59kD
• APPBP1
• APP-BP1
• HPP1
• NAE1
• NEDD8 activating enzyme E1 subunit 1
• NEDD8-activating enzyme E1 regulatory subunit
• NEDD8-activating enzyme E1 subunit
• protooncogene protein 1
• Proto-oncogene protein 1
• ula-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAE1