Apolipoprotein H/ApoH Antibody

Western Blot: Apolipoprotein H/ApoH Antibody [NBP1-97764] - NBP1-97764 (0.2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Apolipoprotein H/ApoH Antibody Summary

• Description: Novus Biologicals Goat Apolipoprotein H/ApoH Antibody (NBP1-97764) is a polyclonal antibody validated for use in WB and ELISA. Anti-Apolipoprotein H/ApoH Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PSIPTFATLRVYK corresponding to internal region according to NP_000033.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.2 - 0.6 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human liver, kidney and placenta lysates is reported in scientific literature (PMID: 10235557).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein H/ApoH Antibody

• Activated protein C-binding protein
• Anticardiolipin cofactor
• APC inhibitor
• APOH
• apo-H
• apolipoprotein H (beta-2-glycoprotein I)
• Apolipoprotein H
• B2G1
• B2GP1
• B2GPI
• Beta
• beta(2)GPI
• beta-2-glycoprotein 1
• Beta-2-glycoprotein I
• BG

Background

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein H/ApoH Antibody (NBP1-97764)(2)

Publications using NBP1-97764

• SzabO G, Debreceni IB, Tarr T et al. Anti-beta 2-glycoprotein I autoantibodies influence thrombin generation parameters via various mechanisms Thromb Res 2020-11-06 [PMID: 33212379] (WB, Human)
  
  Details:
  Western blot analysis performed on precipitated proteins from plasma
• Hirose N, Williams R, Alberts AR et al. A role for the polymorphism at position 247 of the beta2-glycoprotein I gene in the generation of anti-beta2-glycoprotein I antibodies in the antiphospholipid syndrome. Arthritis Rheum 1999-08-01 [PMID: 10446865]

Bioinformatics

• Gene Symbol: APOH
• Entrez:
  • 350(Human)
  • 287774(Rat)
• Uniprot:
  • NP_000033.2(Human)